Composite

Part:BBa_K4613303:Design

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)


pET-29a(+)-T3-ADH3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1007
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1742
    Illegal AgeI site found at 1430
    Illegal AgeI site found at 1592
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 75


Design Notes

We attached 6*his tag to the N-terminus of T3-ADH3 to get purified T3-ADH3.


Source

NAU-CHINA 2023

References

  1. Dai Z, Yang X, Wu F, et al.Living fabrication of functional semi-interpenetrating polymeric materials[J].Nat Commun,2021, 12 (1): 3422.
  2. Zakeri B, Fierer J O, Celik E, et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proc Natl Acad Sci U S A,2012, 109 (12): E690-7.
  3. Reddington S C, Howarth M.Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher[J].Curr Opin Chem Biol,2015, 29: 94-9.
  4. Dai L, Niu D, Huang J W, et al.Cryo-EM structure and rational engineering of a superefficient ochratoxin A-detoxifying amidohydrolase[J].J Hazard Mater,2023, 458: 131836.